Table 1.
Quantitative accuracy of the cleavable ICAT reagent in complex samples
| Mixed ratio (light: heavy) | 1:5 | 1:1 | 5:1 |
| Expected | 0.2 | 1.0 | 5.0 |
| Average | 0.168 | 0.882 | 5.41 |
| Median | 0.159 | 0.895 | 5.31 |
| Stdev | 0.088 | 0.142 | 1.24 |
| 95th percentile | 0.227 | 1.093 | 7.73 |
| 5th percentile | 0.103 | 0.657 | 3.63 |
| Variance | 0.008 | 0.020 | 1.53 |
| Variance % | 4.83 | 2.26 | 28.8 |
| Number of proteins analyzed | 146 | 213 | 150 |
Relative abundance ratios of total proteins isolated from N2-fixing filaments of N. punctiforme labeled with the light- or heavy-ICAT reagent and mixed in 1:1, 1:5 and 5:1 ratios.