Table 3.
SW480 bulk chromatin (n) | Recomb. H3 octamer (n) | Recomb. CENP-A octamer (n) | SW480 total CENP-A (n) | SW480 ectopic CENP-A (n) | |
---|---|---|---|---|---|
Height a (nm) | 2.53 ± 0.26 (401) | 2.32 ± 0.32 (57) | 2.37 ± 0.34 (57) | 2.17 ± 0.35 (546) | 2.46 ± 0.40 (135) |
Diameter b (nm) | 13.8 ± 1.4 (401) | 12.1 ± 1.4 (20) | 10.6 ± 1.4 (20) | 14.1 ± 2.9 (546) | 14.5 ± 3.0 (135) |
AFM measurements of aheight and bdiameter data of ectopic versus centromeric or recombinant CENP-A nucleosomes provided as average ± standard deviation, with number of particles measured indicated in parenthesis. On average, each type of experiment has three replicates.