Table 2.
Development rate of buffalo oocytes / embryos under different levels of oxygen (5 and 20 %) and glucose (5.6 and 20 mM)
Oxygen level (%) | Glucose level - IVM (mM) | # COCs | % M-II | % uncleaved oocyte | % 2 cell (blocked) | % 8–16 cell (blocked) | % Morula (blocked) | % Blastocyst |
---|---|---|---|---|---|---|---|---|
20 | 5.6 | 232 | 87.3 ± 2.06a | 33.3 ± 1.46a | 7.52 ± 0.29a | 8.32 ± 0.64a | 29.17 ± 1.22a | 21.66 ± 0.15a |
5 | 5.6 | 216 | 80.69 ± 0.1a | 30.45 ± 2.5a | 8.42 ± 0.87a | 14.5 ± 2.18b | 28.23 ± 1.38a | 18.32 ± 1.37a |
5 | 20 | 243 | 94.8 ± 2.6b | 25.3 ± 2.87a | 4.19 ± 0.93b | 5.74 ± 1.60c | 35.80 ± 4.52b | 28.96 ± 0.91b |
Values indicate Mean ± SEM. Different superscripts indicate significant difference along column (p < 0.05). Glucose concentration at IVC-I and –II was kept at 5.6 mM both at 5 and 20 % O2