Table 1.
List of proteins with different abundance in multidrug-resistant and susceptible M. tuberculosis isolates (q value for all was <0.05)
| Rv number | Proteins description | Gene name | Functional categoriesa | Fold difference |
|---|---|---|---|---|
| Higher abundance in resistant isolates | ||||
| Rv1398c | Putative antitoxin | vapB10 | 0 | 2.7 |
| Rv3648c | Probable cold shock protein A | cspA | 0 | 2.8 |
| Rv0470c | Cyclopropane mycolic acid synthase 3 | pcaA | 1 | 4.3 |
| Rv2187 | Long-chain-fatty-acid–CoA ligase | fadD15 | 1 | 4.5 |
| Rv1912c | Possible oxidoreductase | fadB5 | 1 | 5.9 |
| Rv2831 | Enoyl-CoA hydratase/isomerase family protein | echA16 | 1 | 6.2 |
| Rv1080c | Transcription elongation factor | greA | 2 | 2.0 |
| Rv1234 | Probable transmembrane protein | 3 | 2.1 | |
| Rv3690 | Probable conserved membrane protein | 3 | 2.1 | |
| Rv1249c | Possible membrane protein | 3 | 2.6 | |
| Rv3794 | Probable arabinosyltransferase A | embA | 3 | 2.9 |
| Rv2873 | Cell surface lipoprotein | mpt83 | 3 | 3.7 |
| Rv2945c | Putative lipoprotein | lppX | 3 | 4.2 |
| Rv1899c | Uncharacterized protein | 3 | 4.7 | |
| Rv0341 | Isoniazid-induced protein | iniB | 3 | 4.8 |
| Rv3841 | Ferritin | bfrB | 7 | 2.1 |
| Rv2344c | Deoxyguanosinetriphosphate triphosphohydrolase-like protein | dgt | 7 | 2.2 |
| Rv2971 | Uncharacterized oxidoreductase | 7 | 2.9 | |
| Rv2713 | Probable soluble pyridine nucleotide transhydrogenase | sthA | 7 | 2.9 |
| Rv2858c | Probable aldehyde dehydrogenase | aldC | 7 | 3.5 |
| Rv0392c | NADH dehydrogenase | ndh-1 | 7 | 9.0 |
| Rv2259 | S-nitrosomycothiol reductase | mscR | 7 | 536 |
| Rv3133c | Transcriptional regulatory protein | devR | 9 | 2.6 |
| Rv0566c | UPF0234 protein | 10 | 2.2 | |
| Rv1156 | Conserved protein | 10 | 2.6 | |
| Rv1770 | Conserved protein | 10 | 3.0 | |
| Rv0577 | 27 kDa antigen | cfp30B | 10 | 3.0 |
| Rv2337c | Uncharacterized protein | 10 | 3.3 | |
| Lower abundance in resistant isolates | ||||
| Rv0456c | Enoyl-CoA hydratase | echA2 | 1 | 13.4 |
| Rv0672 | Acyl-CoA dehydrogenase, putative | fadE8 | 1 | 3.1 |
| Rv2524c | Probable fatty acid synthase | fas | 1 | 2.1 |
| Rv1708 | Uncharacterized protein | 3 | 11.8 | |
| Rv0347 | Probable conserved membrane protein | 3 | 4.5 | |
| Rv3868 | ESX-1 secretion system protein | eccA1 | 3 | 3.4 |
| Rv1038c | Putative ESAT-6-like protein | 3 | 3.3 | |
| Rv3620c | Putative ESAT-6-like protein 10 | 3 | 3.3 | |
| Rv3781 | O-antigen export system | rfbE | 3 | 2.9 |
| Rv1197 | Putative ESAT-6-like protein | 3 | 2.9 | |
| Rv2347c | Putative ESAT-6-like protein 7 | 3 | 2.9 | |
| Rv1793 | ESAT-6-like protein | esxN | 3 | 2.6 |
| Rv0932c | Phosphate-binding protein | pstS2 | 3 | 2.5 |
| Rv2462c | Trigger factor | tig | 3 | 2.4 |
| Rv2155c | UDP-n-acetylmuramoylalanine–d-glutamate ligase | murD | 3 | 2.4 |
| Rv2691 | CeoB | ceoB | 3 | 2.3 |
| Rv1368 | Putative lipoprotein | lprF | 3 | 2.2 |
| Rv1926c | Immunogenic protein | mpt63 | 3 | 2.0 |
| Rv3614c | ESX-1 secretion-associated protein | espD | 3 | 2.0 |
| Rv2964 | Formyltetrahydrofolate deformylase | purU | 7 | 43.9 |
| Rv2280 | Uncharacterized FAD-linked oxidoreductase | 7 | 13.6 | |
| Rv2161c | Conserved protein | 7 | 13.5 | |
| Rv2952 | Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase | 7 | 11.4 | |
| Rv3086 | Putative alcohol dehydrogenase d | adhD | 7 | 9.1 |
| Rv2391 | Sulfite reductase [ferredoxin] | sir | 7 | 5.4 |
| Rv2682c | 1-deoxy-d-xylulose-5-phosphate synthase | dxs | 7 | 4.8 |
| Rv3710 | 2-isopropylmalate synthase | leuA | 7 | 4.5 |
| Rv1895 | Probable zinc-binding alcohol dehydrogenase | 7 | 4.1 | |
| Rv3257c | Phosphomannomutase | manB-1 | 7 | 3.5 |
| Rv2363 | Putative amidase | amiA2 | 7 | 2.3 |
| Rv0814c | Conserved protein | sseC1 | 7 | 2.2 |
| Rv3118 | Uncharacterized protein | sseC1 | 7 | 2.2 |
| Rv3324c | Cyclic pyranopterin monophosphate synthase accessory protein 3 | moaC3 | 7 | 2.1 |
| Rv0844c | Probable transcriptional regulatory protein | narL | 9 | 43.6 |
| Rv2159c | Conserved protein | 10 | 14.3 | |
| Rv0825c | Conserved protein | 10 | 10.2 | |
| Rv3168 | Putative aminoglycoside phosphotransferase | 10 | 4.1 | |
| Rv1444c | Uncharacterized protein | 10 | 2.5 | |
| Rv2623 | Universal stress protein | 10 | 2.5 | |
| Rv3169 | Conserved protein | 10 | 2.1 | |
aFunctional categories 0—virulence, detoxification, adaptation; 1—lipid metabolism; 2—information pathways; 3—cell wall and cell processes; 7—intermediary metabolism and respiration; 9—regulatory proteins; 10—conserved hypotheticals