Print screen or screen snip pictures of representative Mca1 measurements from (A) ASH, (B) NASH, and (C) normal control liver samples. The presence of Mca1 was detected by the green immunofluorescence on the liver biopsy picture. A yellow tracer line converted this fluorescence intensity into quantitative figures and represented it as a green line on a graph, shown below each picture. The level of fluorescence intensity is dependent on the amount of Mca1. (A) ASH showed more green fluorescence compared to both (B) NASH, and (C) normal control liver biopsies. More fluorescence corresponded to the increase presence of Mca1. (D) Means with SD of Mca1 in ASH, NASH, and normal control liver. Levels of Mca1 in ASH are significantly elevated (p<0.05) compared to the normal control liver. There is no significant difference in the levels of Mca1 in NASH versus normal control liver. Comparing ASH versus NASH, data showed that Mca1 is significantly elevated in ASH (p<0.01). (×566 magnification)