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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mucosal Immunol. 2014 Sep 17;8(3):516–532. doi: 10.1038/mi.2014.85

Figure 4.

Figure 4

Food allergic responses are significantly reduced in IgER-TG animals. (a) Decreased mast cell-specific transcripts (i.e. mast cell proteases MCPT1, MCPT2, and carboxypeptidase A3 (CPA3)) and lower IL-4, IL-13, CCL-2, and IL-6 expression were detected in IgER-TGs. Sensitized mice are indicated by (+), non-sensitized mice by (−). Normalized mRNA counts or fold change in mRNA levels +/− SEM of small intestinal tissue of 4 independent experiments (per experiment tissue of ≥2 mice was pooled) are shown. (b) and (c) Small intestinal tissue sections were stained, and infiltrating mast cells were counted. (d) Assessment of mast cell protease MCPT1 in serum as a systemic readout for IgE-mediated mast cell activation. Serum was collected 1h after oral challenge. Symbols are representative of individual mice (n=2).