Fig 2. Effect of pH and temperature on YlLac activity.
(a) Effect of pH on the activity of purified YlLac towards ABTS (●), toluidine (▼), guaiacol (Δ), and 2,6-DMP (○). Reactions were at room temperature for 3 min in citrate/acetate/phosphate buffer. (b) Effect of temperature on the activity of purified YlLac assessed by the standard assay method. Activities are expressed as percentages of maximum activity; the error bars do not exceed the dimensions of the symbols.