Fig. 6. Immunochemical image for confirmation of reduced Bax expression by DHA treatment. (A) Immunochemical images showed that Bax positive cells (red) were densely expressed in the EC group. In DHA treatment group, Bax expression was decreased in rat cortex, compared with the EC group. (B) In DHA treatment group, Bax positive cells were decreased in rat striatum due to DHA treatment. (C) The graph showed the percentage (%) of Bax positive cells to compare the difference of Bax fluorescence intensity. Statistical significance with EC group was determined by t-test. (D) The graph of Bax protein level showed the same pattern with immunochemical images. Differences were considered significant at ^*p<0.05, ^**p<0.01 (ANOVA followed by Bonferroni post hoc multiple comparison test). Scale bar=100 µm, Bax: bcl2 associated X protein (Bax); red, 4', 6-diamidino-2-phenylindole (DAPI): blue. NC: normal control group, EC: experimental control; reperfusion 24 hr after MCAO injury, DHA: DHA treatment and reperfusion 24 hr after MCAO injury.