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. 2015 Mar 14;11:19. doi: 10.1186/s13007-015-0062-x

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© Galindo-González et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sequence coverage and frequency of variants in gene sections of the pilot experiment. DNA from the cultivar Macbeth was diluted (1:64 or 1:96) in CDC Bethune DNA in several simulated pools as described in methods. Each line in the sequence coverage graphs represents one of 28 pools. The frequencies of the variants are plotted against the position in the respective reference sequence section. Each symbol in the frequency graphs represents a frequency of at least 0.1% for each of the 28 pools in each position. A. S20, B. S900; no graphs for S411 are shown since no variants were detected in that sequence.