Figure 5.
A G-protein-dependent transduction mechanism. A, Thirty minutes after establishing the whole-cell configuration, application of 10 μm 5-HT evoked a response with intracellular perfusions of 1.5 mm GTP-γ-S, 10 mm BAPTA, 10 μm PKC19–31 and 1 mm SQ22536. B, Pooled data for the 5-HT-evoked current under normal internal recording solution (n = 35) or during intracellular dialysis of GTP-γ-S (n = 7), BAPTA (n = 5), PKC19–31 (n = 6), and SQ22536 (n = 5). Bars represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired t test).