Figure 5.
Effects of rapamycin treatment on S6K1 and S6 phosphorylation in the cerebellum of WT and AS mice. A, C, Representative images of Western blot processed for p-S6K1 (389), S6K1, p-S6 (235/236), p-S6 (240/244), and S6 in P2 (A) and S2 (C) fractions. B, D, Quantitative analysis of Western blots shown in A and C (means ± SEMs from 3–5 mice; *p < 0.05, **p < 0.01, ***p < 0.001 compared with vehicle-treated WT mice; #p < 0.05, ##p < 0.01, ###p < 0.001 compared with vehicle-treated AS mice, two-way ANOVA with Newman–Keuls post hoc test). VEH, Vehicle; RAPA, rapamycin.