FIG 6.

The yersiniabactin receptor is surface exposed and accessible to antibodies. Histogram overlay of FyuA surface expression and antibody binding as assessed by flow cytometry. UPEC strains 536 and 536ΔfyuA were cultured under iron limitation and incubated with an FyuA-specific MAb (5E7.22), followed by a secondary antibody conjugated to FITC. The shaded area represents a control E. coli strain unable to express FyuA (536ΔfyuA), and the unshaded area denotes antibody binding to wild-type E. coli.