FIG 1.

MyD88 is required for host survival after infection with Histoplasma. (A) Gender- and age-matched MyD88^−/−, Dectin-1^−/−, and IL-1R^−/− mice and their respective wild-type counterparts (n = 6 to 10) were infected intranasally with 1.8 × 10⁴ Histoplasma cells and monitored for survival. Differences in survival were determined using a log-rank test. (B and C) Lungs (B) and spleens (C) of infected MyD88^−/− and C57BL/6J mice were harvested, homogenized, and plated for CFU at the indicated time points (n = 5 or 6 mice/time point for each strain; d, days). All results are representative of at least three independent experiments. *, P < 0.05; ***, P < 0.001; N.D., not detectable. P values were determined using ANOVA. Horizontal bars are means of analyzed samples.