FIG 3.

Inflammatory cell recruitment to the lung requires MyD88 signaling. Infected lungs were dissociated into single-cell suspensions and analyzed via flow cytometry to determine the numbers of specific cell populations. (A) Total lung cell counts. (B) CD45⁺ cell counts. (C) Alveolar macrophage count as defined by CD11c⁺ SiglecF⁺ autofluorescent cells. (D) Dendritic cell count as defined by CD11c⁺ CD11b⁺ MHC class II^high cells. (E) Neutrophil count as defined by CD11c⁻ CD11b⁺ Ly6C⁺ Ly6G⁺ cells. (F) Monocyte count as defined by CD11c⁻ CD11b⁺ Ly6C⁺ Ly6G⁻ cells. The bars represent the means ± SD of 5 or 6 mice. All results are representative of at least three independent experiments. **, P < 0.01; ***, P < 0.001; P values were determined using ANOVA.