FIG 6.

MyD88 is required for appropriate cytokine signaling in alveolar macrophages and bone-marrow-derived dendritic cells in vitro. (A and B) BMDCs (A) and alveolar macrophages (AvMs) (B) from WT and MyD88^−/− mice were harvested and infected in vitro with Histoplasma at an MOI of 1. Supernatants from infected cells were collected in triplicate at 48 h and evaluated for cytokine levels. (C) BMDCs infected with wild-type Histoplasma (MOI = 1) were cocultured with purified splenic WT CD4⁺ T cells. The cells were lysed, and CFU were counted at the indicated time points. *, P < 0.05; ***, P < 0.001; P values were determined by ANOVA. The bars represent the means ± SD of 3 or 4 samples. All the results are representative of at least three independent experiments.