FIG 3.

PIK3C2A promotes vacuole-like protrusion (VLP) formation in HT-29 cells. Schematic representation of the dissemination process in mock-treated (A) or PIK3C2A-depleted (B) HT-29 cells. Each line represents the tracking of one bacterium every 5 min for 160 min, and the progression of the dissemination process was depicted using the color key displayed at the bottom of the panels: protrusion, light blue; VLP, purple; vacuole, yellow; free bacteria in the cytosol of adjacent cells, red; retraction to the primary infected cells, dark blue. Thirty bacteria were tracked in 10 independent infection foci.