FIG 2.
Expression of zebrafish pcsk genes in furinAtd204e/+ mutants and WT controls. (A) Relative furinA expression was measured with qRT-PCR in the tissue homogenates of organs in the abdominal cavity (n = 10) and kidney (n = 10) as well as in purified granulocytes (n = 5) and lymphocytes (n = 5) isolated from steady-state WT AB zebrafish. Samples were run as technical duplicates. (B) The relative expressions of zebrafish pcsk genes (pcsk1, pcsk2, furinB, pcsk5a, pcsk5b, and pcsk7) were quantified in the tissue homogenates of organs in the abdominal cavities of steady-state adult furinAtd204e/+ mutant (n = 10) and WT (n = 8 to 10) zebrafish by using qRT-PCR. Gene expressions were normalized to eef1a1l1 expression and are represented as a scatter dot plot and median. A two-tailed Mann-Whitney test was used in the statistical comparison of differences.