FIG 5.
Reduced Furin expression is associated with an upregulated expression of Tnf in activated mouse macrophages. Bone marrow-derived macrophages were cultured from Furin KO (LysMcre-furfl/fl) and WT littermate mice (n = 4 to 5) as described previously (46). The relative expressions of Furin (Ensembl ID ENSMUSG00000030530) (A) and Tnf (Ensembl ID ENSMUSG00000024401) (B) were determined in unstimulated and LPS-stimulated (4 h) samples with qRT-PCR. Gene expressions were normalized to ribosomal protein S18 (Rps18, Ensembl ID ENSMUSG00000008668) expression and are represented as a scatter dot plot and median. A two-tailed Mann-Whitney test was used in the statistical comparison of differences. The qRT-PCR primers used for the mouse genes were as follows: Furin, 5′-CAGAAGCATGGCTTCCACAAC-3′ and 5′-TGTCACTGCTCTGTGCCAGAA-3′; Tnf, 5′-CTTCTGTCTACTGAACTTCGGG-3′ and 5′-CAGGCTTGTCACTCGAATTTTG-3′; and Rps18, 5′-GTGATCCCTGAGAAGTTCCAG-3′ and 5′-TCGATGTCTGCTTTCCTCAAC-3′.