FIG 2.

SDS-PAGE and Western blotting of major outer membrane proteins. All strains examined in the animal model were separated by SDS-PAGE. SS, Sheila Smith; SP, Sao Paulo; M, Morgan; I, Iowa; HLP, HLP7421. (A) Coomassie staining showing the presence of rOmpA in all strains except Iowa and the 120-kDa rOmpB protein in which the larger unprocessed form is abundant in the Iowa strain. (B) Immunoblot of rOmpA with 13-3 monoclonal antibody. (C) Immunoblot of the 120-kDa rOmpB protein using a rabbit polyclonal anti-R. rickettsii rOmpB antiserum. In the Iowa strain, both the processed and larger, unprocessed forms are detected.