FIG 2.

Transferring CAP60Δ/DCs ameliorates pulmonary infection with highly virulent C. gattii strain R265. CAP60Δ/DCs or unloaded DCs were injected into tail veins of mice at 14 days and 1 day prior to intratracheal infection with 3 × 10³ CFU of strain R265. (A and B) Fungal burdens (n = 5) in the lungs were determined at day 14 (A) and day 3 (B). Pooled data from two independent experiments are plotted, and median values are shown by short horizontal lines. *, P < 0.05 by analysis of variance with Dunn's post hoc test (A) or P < 0.05 by Mann-Whitney U test (B). (C) Survival curves (n = 8) were compared by log rank test. Representative data from two independent experiments are shown. *, P < 0.01. Median survival times were 28 days in the case of nontransfer and 238 days in the case of CAP60Δ/DC transfer.