FIG 4.

Role of HptRS in intracellular survival/multiplication of S. aureus. A cell monolayer of murine bone marrow-derived macrophages (BMDM), human monocytic cells (THP-1), and human alveolar epithelial cells (A549) was infected with the wild-type or ΔHptRS strains at an MOI of 5. After 30 min of infection, extracellular bacteria were removed by gentamicin treatment, and survival/multiplication of S. aureus within the host cells were measured at the indicated time points. Results shown are combined from triplicate measurements from three independent experiments. The asterisk indicates statistical difference from the wild type at P values of <0.05.