TABLE 1.
Strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| S. aureus | ||
| RN4220 | Cloning intermediate derived from 8325-4 | 45 |
| USA300 LAC | USA300 wild-type reference strain | |
| ΔHptA mutant | SAUSA300_0219, hptA deletion mutant | This study |
| ΔHptRS mutant | SAUSA300_0217 and 0218, hptR hptS double deletion mutant | This study |
| ΔUhpT mutant | SAUSA300_0216, uhpT deletion mutant | This study |
| ΔHptA-Comp mutant | LAC ΔHptA mutant complemented with pMK4-HptA | This study |
| ΔHptRS-Comp mutant | LAC ΔHptRS mutant complemented with pMK4-HptRS | This study |
| ΔUhpT-Comp mutant | LAC ΔHptA mutant complemented with pMK4-UhpT | This study |
| E. coli DH5α | Cloning intermediate | 46 |
| Plasmids | ||
| pMAD-CM-GFPuv | Allelic replacement temperature-sensitive vector | This study |
| pMK4 | E. coli-S. aureus shuttle vector | 26 |
| pMK4-HptA | pMK4 harboring complementation of the hptA deletion | This study |
| pMK4-HptRS | pMK4 harboring complementation of the hptR and hptS deletions | This study |
| pMK4-UhpT | pMK4 harboring complementation of the uhpT deletion | This study |