FIG 5.
Outcome of infection of mice with C. rodentium strains. (A) Kaplan-Meier survival curve of 3-week-old mice infected with the C. rodentium ΔregA strain (EMHI) carrying the empty vector pACYC184, pAT9 (RegASMS), or pAT6 (RegACR) or wild-type C. rodentium (ICC169) carrying pACYC184. The data were collected over 20 days from 6 mice in each group. (B) Colonic-crypt height on day 10 of infection of 4-week old mice, showing the value for each mouse and the mean of each experimental group derived from at least 8 full-height measurements from five mice in each group. Differences in crypt heights were analyzed by ANOVA with the Tukey posttest. ns, P > 0.05; ***, P < 0.001. (C) Representative pathology of mouse colons on day 10 of infection. The sections show crypt height in mice infected with the C. rodentium ΔregA strain (EMHI) carrying the empty vector pACYC184 (i), pAT9 (RegASMS) (ii), or pAT6 (RegACR) (iii) or wild-type C. rodentium carrying pACYC184 (iv). The sections were stained with hematoxylin and eosin. Bar, 200 μm.