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. 2015 Mar 17;83(4):1347–1353. doi: 10.1128/IAI.00032-15

FIG 2.

FIG 2

Specificity of PCR oligonucleotide primer pairs to detect wild-type (297) and luxS mutant (AH309) B. burgdorferi strains. (A) Schematic representation of locations of sequences complementary to PCR primers. Primers ermC-F and ermC-R both correspond to sequences within the ermC gene that is inserted into luxS of AH309 (22). Oligonucleotide luxS-R overlaps the ermC insertion site of the AH309 locus and thus cannot serve to prime PCR for that strain. (B) Purified genomic DNAs from strains 297 and AH309 were subjected to PCRs using each primer pair and then subjected to agarose gel electrophoresis and ethidium bromide staining. Lane 1, 297 with primers luxS-F and luxS-R; lane 2, 297 with primers ermC-F and ermC-R; lane S, molecular size markers; lane 3, AH309 with primers luxS-F and luxS-R; lane 4, AH309 with primers ermC-F and ermC-R. Sizes of markers are indicated to the right of the gel.