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qPCR analyses of tissues from mice that were simultaneously injected with 104 bacteria (each) of both strains 297 and AH309. Total DNAs were extracted from the hearts, urinary bladders, and ear pinnae of eight mice. Ratios of wild-type (strain 297) to luxS mutant (strain AH309) bacteria were calculated from the ΔCT values for each strain in each tissue. Competition indexes were calculated for every mouse tissue.
