TABLE 1.
Oligonucleotide primers used for PCR
| Purpose | Primer name | Primer sequence (5′ to 3′)a |
|---|---|---|
| Detection of mouse chromosomes | nido-F | CCAGCCACAGAATACCATCC |
| nido-R | GGACATACTCTGCTGCCATC | |
| Detection of B. burgdorferi chromosomes | flaB-F | GGAGCAAACCAAGATGAAGC |
| flaB-R | TCCTGTTGAACACCCTCTTG | |
| Detection of wild-type luxS | luxS-F | GAGCACATAGGAGCTACTTTACTT |
| luxS-R | TGAGACTAAGTCAACAAGATC-TTTAC | |
| Detection of AH309 mutant luxS locus | ermC-F | AAACGCTCATTGGCATTACTTT |
| ermC-R | TGAGCTATTCACTTTAGGTTTAGGA |
a
The dash in the luxS-R sequence indicates the point of luxS into which the ermC gene was inserted to create mutant strain AH309. Due to the split of the luxS-R target sequence in AH309, that oligonucleotide cannot serve as a PCR primer for AH309 (see Fig. 2).