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Transcriptional analysis of the secA gene in ATCC 19606T and 2010 cells. qRT-PCR was used to detect secA transcripts produced in the ATCC 19606T parental strain and the isogenic 2010 secA insertion mutant using the primers 3976 and 3977, which anneal to the 5′ end of the this gene, using recA expression for normalization. The error bars represent the standard error (SE) of the mean of data collected from three independent biological samples.
