TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Relevant characteristic(s)a	Source or reference
Strains
A. baumannii
19606T	Clinical isolate, type strain	ATCC
19606T 2010	secA::EZ::TN<R6Kγori/KAN-2> derivative of 19606T; Kmr	This work
19606T 2010.C	2010 derivative harboring pMU472, parental iron utilization phenotype; Kmr Ampr	This work
19606T s1	basD::aph, 19606T acinetobactin-production deficient derivative; Kmr	10
19606T t6	bauA::EZ::TN<R6Kγori/KAN-2> derivative of ATCC 19606T, acinetobactin-utilization deficient; Kmr	10
E. coli
EC100D	pir+, used for plasmid rescue	Epicentre
Top10	Used for DNA recombinant methods	Life Technologies
BL21 Star (DE3)	λDE3, T7 RNA polymerase	Life Technologies
Plasmids
pCC1	PCR cloning vector; Cmr	Epicentre
pCR-Blunt II-TOPO	PCR cloning vector; Kmr Zeor	Life Technologies
pET100D	T7 expression vector; Ampr	Life Technologies
pET200D	T7 expression vector; Kmr	Life Technologies
pUC4K	Source of aph encoding Kmr	Pharmacia
pWH1266	A. baumannii-E. coli shuttle vector; Ampr Tetr	32
pMU450	Plasmid rescued by self-ligation of EcoRI-digested DNA from mutant 2010; Kmr	This work
pMU451	Plasmid rescued by self-ligation of NcoI-digested DNA from mutant 2010; Kmr	This work
pMU468	pCR-Blunt II-TOPO harboring secA from 19606T; Kmr	This work
pMU472	pWH1266 harboring secA from 19606T; Ampr Tets	This work
pMU481	pET200D harboring secA from 19606T; Kmr	This work
pMU734	1.3-kbp secA internal amplicon from 19606T cloned in pCC1	This work
pMU884	pET100D harboring bauB internal amplicon from 19606T; Ampr	This work
pMU887	pET100D harboring bauE internal amplicon from 19606T; Ampr	This work

^aAmp^r, ampicillin resistance; Km^r, kanamycin resistance; Cm^r, chloramphenicol resistance; Zeo^r, zeocin resistance; Tet^r, tetracycline resistance; Tet^s, tetracycline sensitivity.