TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic | Reference or source |
|---|---|---|
| Strains | ||
| E. coli DH5α | Laboratory strain K-12, recA negative | Gibco-BRL |
| E. coli DH5αΔbaeSR::tet | Strain where BaeSR has been replaced by tet | 19 |
| E. coli DH5αΔbaeSR::tet(pGEM::baeSR) | DH5αΔbaeSR complemented with baeSR | This study |
| E. coli DH5αΔbaeSR::tet(pGEM) | DH5αΔbaeSR complemented with pGEM-T Easy without baeSR | This study |
| E. coli C600 (933W) | Lysogen with phage 933W | 13 |
| E. coli C600 (933WΔstx2::cat) | Lysogen with recombinant phage 933W | 15 |
| E. coli C600 (ΦA9Δstx2::cat) | Lysogen with recombinant phage ΦA9 | 15 |
| E. coli DH5α (ΦVTB55Δstx2::cat) | Lysogen with recombinant phage ΦVTB55 | 15 |
| E. coli DH5α (ΦA75Δstx2::cat) | Lysogen with recombinant phage ΦA75 | 15 |
| E. coli DH5α (ΦA312Δstx2::cat) | Lysogen with recombinant phage ΦA312 | 15 |
| E. coli DH5α (ΦA534Δstx2::cat) | Lysogen with recombinant phage ΦA534 | 15 |
| E. coli DH5α (ΦA549Δstx2::cat) | Lysogen with recombinant phage ΦA549 | 15 |
| E. coli DH5α (ΦA557Δstx2::cat) | Lysogen with recombinant phage ΦA557 | 15 |
| Plasmids | ||
| pKD46 | Containing Red recombinase system | GenBank AY048746; 18 |
| pKD3 | Source of chloramphenicol resistance gene (cat) | GenBank AY048742; 18 |
| pACYC184 | Source of tetracycline resistance gene (tet) | GenBank X06403; 38 |
| pGEM-T Easy | High-copy-number plasmid | Promega |
| pGEM-T Easy::baeSR | Containing baeSR gene | This study |