FIG 5.

Adhesion of LF82 Δnrd mutants using T-84 cells or ileal loops. (A) Cell-associated bacteria were quantified using undifferentiated T-84 cells after a 3-h infection. (B) Competitive indexes of LF82 with LF82 ΔnrdR, LF82 ΔnrdD, and LF82 ΔnrdE. Intestinal ileal loops were inoculated with a mixed inoculum comprising equivalent numbers of two bacterial strains. After 5 h, the bacterial presence was compared using competitive index analysis, which provides a sensitive measurement of the relative degree of attenuation. *, P < 0.05.