TABLE 1.
Bacterial strains and plasmid used in this study
| Bacterial strain or plasmid | Characteristicsa | Source or referenceb |
|---|---|---|
| B. abortus strains | ||
| B. abortus S2308 | Wild type, smooth | LIDI |
| B. abortus S2308(pBBR4-gfp) | Wild type expressing GFP, smooth | LIDI |
| B. abortus S19 | Vaccine strain, smooth | LIDI |
| B. abortus RB51 | Vaccine strain, rough | LIDI |
| B. abortus Δmfp::kan | Kanr, mutant of S2308, smooth | CICVyA-INTA |
| B. abortus Δmfp(pBBR4-gfp) | Kanr Ampr, mutant of S2308, smooth | This study |
| B. abortus Δmfp(pBBR1-mfp) | Kanr Cmr, mutant of S2308, smooth | CICVyA-INTA |
| B. abortus Δmfp(pBBR1-mfp, pBBR4-gfp) | Kanr Cmr Ampr, mutant of S2308, smooth | This study |
| B. abortus Δomp19::kan | Kanr, mutant of S2308, smooth | CICVyA-INTA |
| B. abortus Δomp19(pBBR4-gfp) | Kanr Ampr, mutant of S2308, smooth | This study |
| B. abortus Δomp19(pBBR1-omp19) | Kanr Cmr, mutant of S2308, smooth | CICVyA-INTA |
| B. abortus Δomp19 (pBBR1-omp19, pBBR4-gfp) | Kanr Cmr Ampr, mutant of S2308, smooth | This study |
| E. coli strains | ||
| E. coli BL21 | Competent strain for protein expression | LIDI |
| E. coli S17 | Competent conjugative donor strain | LIDI |
| Plasmid | ||
| GFP | pBBR4-gfp Ampr | DNA 2.0 |
a
Kanr, kanamycin resistance; Ampr, ampicillin resistance; Cmr, chloramphenicol resistence.
b
LIDI, Laboratório de Imunologia de Doenças Infecciosas, UFMG, Belo Horizonte, Brazil; CICVyA-INTA, Instituto de Biotecnología, CICVyA-INTA, Buenos Aires, Argentina (Silvio L. Cravero).