FIG 1.

Anti-FHbp binding in relation to complement-mediated bactericidal activity. (A and B) Binding of MAb (10 μg/ml) to live bacteria as measured by flow cytometry. Solid black line, resistant (R) isolates; dashed black line, control sensitive (S) isolates. (A) Anti-FHbp MAb JAR 5; (B) anticapsular MAb SEAM 12. Representative data are shown. The results were replicated in two experiments. (C) Complement-mediated bactericidal activity. Anti-FHbp, antisera from mice immunized with an FHbp sequence variant that matched the FHbp amino acid sequence variant of the respective isolates. Anti-capsule, anticapsular MAb SEAM 12. The data are calculated from results of three assays. Error bars represent ranges.