FIG 3.

Effect of knocking out NspA on Anti-FHbp bactericidal activity. (A) Binding of anti-NspA MAb to live bacteria. Anti-NspA MAb 14C7 was tested at 10 μg/ml. Black solid line, resistant (R) strains; dashed black lines, susceptible (S) strains; gray-filled histogram, mutants of R and S isolates with nspA inactivated. Typical results shown; data were replicated in two experiments. (B and C) Complement-mediated bactericidal activity. Mouse anti-FHbp antisera and anticapsular MAb are described in the legend for Fig. 1B and C, respectively. Error bars represent ranges of duplicate or triplicate measurements in three experiments.