FIG 8.

Effect of allelic replacement of PorB3 variant on anti-FHbp bactericidal activity. The sera were from mice immunized with recombinant FHbp that matched the sequence variant of the isolates (FHbp ID 4). (A) Isolate R1 expressing wild-type FHbp and NspA with either allelic exchange of PorB3 R1 (open bars) or PorB3 S1 (hatched bars); (B) isolate S1 expressing wild-type FHbp and NspA with either allelic exchange of PorB3 R1 or PorB3 S1. The data were calculated from three assays. Error bars represent ranges. Asterisks indicate that a difference in bactericidal activity between respective PorB3 variants was significant (P = 0.002, Mann-Whitney).