TABLE 1.
Neisseria meningitidis serogroup B strains
| Isolate no.a | Original strain designation | Geographic location | Clonal complex (MLSTb) | PorA VR (VR1, VR2)c | PorB3 variantd (GenBank accession no.) | FHbp amino acid sequence (ID)e | LOS immunotypef |
|---|---|---|---|---|---|---|---|
| R1 | N02/06 | Norway | 41-44 (5780) | 7-2, 4 | 3-90 (KJ946412) | 4 | 8-1 |
| S1 | MD1327 | Maryland | 41-44 (2973) | 18, 25 | 3-ND (KJ946413) | 4 | 8-1 |
| R2 | SK50 | Ohio | 35 (5744) | 15-1, 3 | 3-7 (KJ997945) | 13 | 3-7-9 |
| S2 | SK57 | Tennessee | 213 (213) | 22, 14 | 3-14 (KJ946414) | 13 | 3-7-9 |
R, anti-FHbp-resistant isolates (titers < 1:10); S, anti-FHbp sensitive isolates (titers ≥ 1:1,000 [see Results]).
MLST, multilocus sequence type. Data are presented at http://pubmlst.org/neisseria/.
Based on PorA variable region (VR) sequence types presented at http://pubmlst.org/neisseria/.
All strains had PorB3 alleles inferred from DNA sequencing and as designated at http://pubmlst.org/neisseria/. 3-ND, not designated in database, but the sequence is one amino acid different from “3-16.”
All strains had FHbp in subfamily B (variant group 1) and were assigned to amino acid sequence variant ID 4 or 13, as indicated at http://pubmlst.org/neisseria/.
Based on reactivity with specific anti-LOS MAbs anti-L3,7,9, anti-L1, or anti-L8 (see Materials and Methods). The isolates were grown in broth containing CMP-NANA; R2 and S2 expressed sialylated LOS based on lack of binding by MAb 3F11 (see Materials and Methods).