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. 2015 Mar 17;83(4):1715–1731. doi: 10.1128/IAI.03155-14

FIG 4.

FIG 4

IFA micrographs of N. bombycis collected from different Percoll gradient fractions. N. bombycis was incubated with anti-SWP9 (A3, B3, C3, D3, and E3) labeled with FITC (green) and anti-SWP7 (A4, B4, C4, D4, and E4) labeled with Alexa Fluor 647 (red). Spores were visualized with a differential interference contrast (DIC) microscope (A1, B1, C1, D1, and E1) and stained with DAPI (A2, B2, C2, D2, and E2). The images of the 30% and 45% Percoll gradient centrifugation fractions show internal spot signals (arrows). (A2 and B2) At the same time, the nuclei of the sporonts were unstained with DAPI. (C5 and D5) Images from the 60% and 75% Percoll gradient centrifugation fractions show that these fractions are rich in early sporoblasts and late sporoblasts, with fluorescent ring signals and developing walls (arrows). The 90% Percoll gradient centrifugation fraction contains mature spores with intense fluorescent ring signals that are marked by a typical and intact thick wall. Note the presence of both red and green signals inside the spore and around the spore wall and areas of yellow signal where they overlap (arrows).