FIG 7.

Binding of rSWP7-His to DCSCs depends upon the combination of rSWP9-GST and DCSCs. (A) IFA of DCSCs bound by rSWP9-GST, rSWP7-His, and rGST proteins. (A2, B2, and D2) DCSCs were visualized using a fluorescence microscope after incubating the DCSCs with recombinant proteins and primary antibodies against SWP9, SWP7, and GST. (C2) DCSCs were incubated with rSWP7-His after incubating the DCSCs with rSWP9-GST, removing the unbound rSWP9-GST and rSWP7-His with PBS, and incubation with the anti-SWP7 primary antibody. (D2) DCSCs were incubated with rGST using anti-GST as a control. (A1, B1, C1, and D1) Fluostain I was used to stain the chitin component. (B) Immunoblotting analysis to detect whether rSWP9-GST and rSWP7-His proteins bind to DCSCs using anti-SWP9 and anti-SWP7 antibodies. In addition, rGST was used as a negative control. Lanes: S0, recombinant-protein supernatant after incubation with DCSCs; S, supernatant after six washes, indicating DCSCs bound by recombinant protein; P, DCSC pellet bound by recombinant protein after six washes; M, EasySee Western marker.