FIG 8.

SWP9 and SWP7 can attach to BmE host cells. (A) IFA of the BmE host cell bound by total mature-spore proteins and recombinant proteins. (A3, B3, C3, D3, and E3) BmE cells were visualized using a fluorescence microscope after incubation with native total mature-spore proteins or recombinant proteins and primary antibodies against SWP9, SWP7, and GST. (C3 and F3) BmE cells were incubated with rGST or total mature-spore proteins using anti-GST (C3) or negative-control antibodies (F3) as controls and showed no fluorescence at the cell membrane. (A2, B2, C2, D2, E2, and F2) DAPI was used to stain the nuclei. All images, ×1,000 magnification. (B) Anti-SWP9 and anti-SWP7 antibodies were used for Western blot analysis to detect rSWP9-GST and rSWP7-His and native SWP9 and SWP7 binding to BmE cells. BmE cells were incubated with rGST or PBS, using anti-GST or anti-SWP9 and SWP7 antibodies as negative controls. Lanes M, EasySee Western marker.