FIG 9.

SWP9 and SWP7 contribute to N. bombycis adherence to and infection of the host cell. (A) Exogenous rSWP9-GST or anti-SWP9 and anti-SWP7 antibodies inhibited spore adherence to the host cell. SWP9- and SWP7-specific antibodies, individually and in combination, or a control preimmune antibody was incubated in an adherence assay with N. bombycis spores at a concentration of 5 μg/ml. Furthermore, rSWP9-GST and control rGST were also used as inhibitors of N. bombycis spore adherence at a concentration of 5 μg/ml. The data are shown as the percentage of adherence (compared to control samples) with preimmune antibody or rGST. (B) Exogenous rSWP9-GST or anti-SWP9 and SWP7 antibodies inhibited spore infection of the host cell. The data are shown as the percentages of infected host cells relative to control samples incubated at the same concentration (the infection percentage of control samples was treated as 100%). The experiments were repeated three times and produced similar results each time. At least 20 random fields were calculated for each data point. The error bars indicate standard deviations (SD).