Figure 2.
(a) Effects of EAE after 10 min pretreatment of the tissues with TTX (1 μM), atropine (10 μM), norepinephrine (1 μM), indomethacin (10 μM), ketotifen (100 μM), cimetidine (10 μM), and pyrilamine (1 μM). EAE (10−8, 10−7, 10−6, 10−5, 10−4, and 10−3 g/mL) was added to the bath solution. (b) Statistical analysis of EAE on the contractility of rat colonic tissue pretreatment with tetrodotoxin (TTX), atropine, norepinephrine (NE), indomethacin (Indo), ketotifen, cimetidine (Cime), and pyrilamine (Pyri), respectively. The mean contractile amplitude of rat colonic tissue in normal contractile state is set as 100% (control). Data are expressed as mean ± SEM (% control, n = 6) * P < 0.05, ** P < 0.01, compared to the control groups.