Figure 5.

EAE-induced histamine release from the rat colonic tissues. (a) Pretreatment of tissues of rat colon with EAE (10⁻⁶ g/mL) increased the concentration of histamine in the supernatant; pretreatment of the tissues with ketotifen (100 μM) prevented EAE-induced histamine release into the supernatant. (b) The histamine concentration in colon tissues treated with EAE was decreased; pretreatment of the colon tissues with ketotifen prevented the EAE-induced reduction in tissue histamine. Data are expressed as mean ± SEM (n = 8) ^** P < 0.01 compared to the control groups.