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Double immunofluorescence staining. An admixture of H-EGFR (a, d, and g; red stain) and H-Beclin1 (b, e, and h; green stain) positive GB cells is observable in areas showing a heterogeneous pattern of protein expression. The merged pictures (c, f, and i), however, show that the positivity is mutually exclusive; a pin point cytoplasmic positivity for Beclin1 is evident in these enlarged details of Figure 2. Scale bar = 25 μm.
