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. 2015 Mar 17;10(3):e0120678. doi: 10.1371/journal.pone.0120678

Fig 1. Isolation of the element responsible for the low copy number limit in the DIE2 region.

Fig 1

  1. Copy number limits of DNA fragments from the DIE2 region. The data were obtained from our previous study [1].
  2. Copy number limits of DNA fragments (Frag5 in A) with serial 10-bp deletions every 100 bp. The asterisk indicates that only single experiment was performed.
  3. Locations of the small ORFs (sORF1 and sORF2) in the 3′ region of DIE2. The numbers indicate the 10-bp deletions analyzed in B. The deletions shown in white did not affect the toxicity of the DNA fragment, whereas the deletion shown in black disrupted the toxicity.
  4. Copy number limits of DNA fragments with ATG to ATC substitutions in sORF2.
  5. Amino acid sequence of sORF2. The substituted methionines (ATG codons) in C are shown in red. A potential NLS sequence is underlined, and an amino acid sequence predicted to construct a helical structure is shown in bold letters.