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. 2015 Mar 17;10(3):e0120678. doi: 10.1371/journal.pone.0120678

Fig 2. Genetic analyses of sORF2.

Fig 2

  1. Overexpression of sORF2 from the GAL1 promoter (P GAL1). The construct used in this experiment is shown. Cells with pTOW-PGAL1-sORF2 (P GAL1 -sORF2) were streaked onto SC-glucose and SC-galactose plates. Two independent plasmid clones were analyzed. pTOW40836 (Vector) was used as an empty vector control and pTOW-PGAL1-GFP (P GAL1 -GFP) was used to monitor the P GAL1 induction.
  2. Time-lapse imaging of cells after the induction of sORF2. The cells with pTOW-PGAL1-sORF2 (P GAL1 -ORF2) and pTOW-PGAL1-GFP (P GAL1 -GFP) were cultured in SC-glucose mixed at a ratio of 10:1 and then cultivated in SC-galactose medium. P GAL1 -GFP was used to monitor the induction of P GAL1. The cellular images shown were obtained every 5min. A movie is available as S1 Movie.
  3. Deletion of sORF2. The construct used to delete sORF2 from the chromosome is shown. The strain with sORF2 deleted was streaked onto a YPD agar plate. The strain BY4741 was used as a wild-type control.