Table 5. Effects of the phytase and Ca/P levels on ileal microbiota in broiler chickens (log cfu/ml digesta).
Treatment | DAPI | BACTO | CPREF | ENTER | LAB | CLEPT | STRC | EREC | BIF |
---|---|---|---|---|---|---|---|---|---|
PC | 9.139 b | 7.933 | 8.103 | 8.225 a | 7.858 b | 7.604 a b | 7.656 a | 7.784 b | 7.915 a |
PC + PHY | 9.592 a | 7.799 | 8.048 | 8.160 a | 8.048 a | 7.571 b | 7.492 b | 7.878 a | 7.628 c |
NC | 8.572 c | 7.942 | 7.868 | 7.985 b | 8.002 a | 7.510 b | 7.567 a b | 7.703 c | 7.934 a |
NC + PHY | 8.978 b | 7.911 | 7.961 | 7.894 b | 8.064 a | 7.675 a | 7.577 a b | 7.704 c | 7.748 b |
Pooled SEM | 0.0467 | 0.0285 | 0.0390 | 0.0268 | 0.0200 | 0.0168 | 0.0159 | 0.0097 | 0.0169 |
Model P | <.0001 | 0.2555 | 0.1558 | <.0001 | 0.0007 | 0.0041 | 0.0034 | <.0001 | <.0001 |
Main Effects | |||||||||
P and Ca level | |||||||||
Optimal | 9.369 a | 7.865 | 8.075 a | 8.192 a | 7.954 b | 7.587 | 7.572 | 7.831 a | 7.769 b |
Deficient | 8.775 b | 7.926 | 7.914 b | 7.939 b | 8.033 a | 7.593 | 7.572 | 7.704 b | 7.841 a |
Phytase | |||||||||
None | 8.851 b | 7.938 | 7.983 | 8.103 | 7.931 b | 7.556 b | 7.611 a | 7.743 b | 7.924 a |
5000 FTU/kg | 9.285 a | 7.855 | 8.004 | 8.027 | 8.056 a | 7.623 a | 7.534 b | 7.791 a | 7.688 b |
P-value | |||||||||
P and Ca level | <.0001 | 0.2870 | 0.0388 | <.0001 | 0.0350 | 0.8806 | 0.9564 | <.0001 | 0.0060 |
PHY | <.0001 | 0.1474 | 0.8069 | 0.1107 | 0.0011 | 0.0407 | 0.0129 | 0.0013 | <.0001 |
Interaction Terms | |||||||||
P and Ca level x PHY | 0.7151 | 0.3679 | 0.3396 | 0.7899 | 0.0943 | 0.0025 | 0.0051 | 0.0015 | 0.0454 |
DAPI – total number of bacteria; BACTO – Bacteroides – Prevotella cluster; CPREF – Clostridium perfringens; ENTER – Enterobacteriaceae; LAB – Lactobacillus sp./Enterococcus sp.; CLEPT – Clostridium leptum subgroup; STRC – Streptococcus/Lactococcus; EREC – Clostridium coccoides—Eubacterium rectale cluster; BIF – Bifidobacterium sp.
PC – positive control (diet adequate in Ca and dP levels); NC – negative control (diet with reduced Ca and dP levels by 0.14 and 0.13% respectively); PC + PHY / NC + PHY – positive/negative control supplemented with 5000 FTU of 6-phytase (EC 3.1.3.26) per 1kg of complete feed
SEM—standard error of the mean
a-cWithin the same column, different superscripts indicate significant differences between treatments (P<0.05)