Table 5.
Serial number | Primer | Sequence | Tm (°C) | Size range |
---|---|---|---|---|
1 | CM11 | F: GCTGCCAGAAAAATGTTGCAGTG | 58 | 270–338 |
R: CTGCCTCGTAAAAGCTTGCGTTG | ||||
| ||||
2 | CM13 | F: GCTATGCAGCTTGTTCGCAATCC | 59 | 350–411 |
R: CCAGCTATATCAGGAGCAGAACC | ||||
| ||||
3 | CM180 | F: CATGTGTAATACATTCAACAGTGA | 60 | 300–350 |
R: GCAATAGTGGTTGTCATCCTT | ||||
| ||||
4 | CM32 | F: AACTCTCCATTCCCGCATTC | 60 | 180–200 |
R: CTGGGTTTTCTGTGTTCTCG | ||||
| ||||
5 | CM46 | F: CAGCTAGTGTGAAGGGAAAC | 55 | 300–350 |
R: GTTATCATGGTCTTACACG | ||||
| ||||
6 | CM20 | F: CTTGTTTGAGTCTGTCGCTG | 60 | 250–300 |
R: TTTCCCTCCCAATGTCTGTA | ||||
| ||||
7 | CM171 | F: TTCCCCCATCTTTTTCTTTC | 60 | 250–300 |
R: TTGTATACGGCTCGTCAGGT | ||||
| ||||
8 | CM166 | F: AAGAGGTGCCTATCACCGTC | 60 | 230–260 |
R: CGAGGTATCAAAAAGCACCT | ||||
| ||||
9 | CM211 | F: TCATGCCAAATATGAGTGGA | 60 | 300–350 |
R: GAGATGGCAAAGGCTGTTC |