Table 1. List of genes and primers used to analyze gene expression by quantitative real time PCR.
| (A) Gene Name | Probe Number | Gene Name | Probe Number |
|---|---|---|---|
| Acp5 (Trap) | Mm00475698_m1 | Bmp2 | Mm01340178_m1 |
| Ccl2 (Mcp1) | Mm00441242_m1 | cFos | Mm00487425_m1 |
| Dkk1 | Mm00438422_m1 | Fgf2 | Mm00433287_m1 |
| Gapdh | Mm99999915_g1 | Igf1 | Mm00439560_m1 |
| Il17a | Mm00439618_m1 | Mepe | Mm02525159_s1 |
| Ramp3 | Mm00840142_m1 | Runx2 | Mm00501584_m1 |
| Sfrp1 | Mm00489161_m1 | Sost | Mm00470479_m1 |
| Tnfα | Mm00443258_m1 | Tnfsf11(Rankl) | Mm00441908_m1 |
| Tnfrsf11b (Opg) | Mm01205928_m1 | Wisp1(Ccn4) | Mm01200484_m1 |
| Wnt4 | Mm01194003_m1 | Wnt10b | Mm00442104_m1 |
| Wnt3a | Mm00437337_m1 | Ptgs2 (Cox2) | Mm00478374_m1 |
| (B) Gene Name | Primer Sequence | ||
| Bglap (Osteocalcin) | Rev TGG TCT GAT AGC TCG TCA CAA G For CTG ACC TCA CAG ATC CCA AGC | ||
| Actin | Rev CCA GTT GGT AAC AAT GCC ATG T For GGC TGT ATT CCC CTC CAT CG | ||
(A) Genes analyzed by TaqMan Gene Expression Assay and the Design Probes used to analyze their expression. (B) Genes analyzed by Syber Green validated primer sequences.