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. 2015 Mar 17;10(3):e0120213. doi: 10.1371/journal.pone.0120213

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© 2015 Ott et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A-F) HeLa cells were grown on cover slips and transfected using Lipofectamine 2000 with pCDNA3 plasmids carrying information for FLAG-tagged Sam50 (A), Mic60/Mitofilin (B), Mic19/CHCHD3 (C), Mic25/CHCHD6 (D), Mic27/ApoOL (E) or Mic23/ApoO (F). Cells were then labeled with MitoTracker (red channel), fixed and immunostained stained using antibodies directed against the FLAG-tag (green channel) or Tom20 (A, blue channel) and corresponding, fluorophore-coupled secondary antibodes. In the upper right corner enlarged sections can be seen. Scale bar represents 10 μm.