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. 2015 Mar 17;10(3):e0120213. doi: 10.1371/journal.pone.0120213

Fig 3. Mitochondrial morphology in knockdown cell lines.

Fig 3

(A-F) Cells from inducible knockdown cell lines, carrying shRNA that downregulates Sam50 (A, sam50kd-2), Mic60/Mitofilin (B, mflkd-2), Mic19/CHCHD3 (C, chchd3kd-2), Mic25/CHCHD6 (D, chchd6kd-3), Mic27/ApoOL (E, apoolkd-2) or Mic23/ApoO (F, apookd-4) were grown on coverslips and induced with doxycycline (Dox) for 7 days. After fixation, mitochondria were decorated with anti-Tom20 antibody and Cy5-coupled secondary antibody. Cells were analyzed by fluorescence microscopy. Enlarged sections of the shown pictures are represented in the upper right corner. Scale bar represents 10 μm. (G) Average circularity as a measurement of mitochondrial fragmentation was analyzed for 10 fields of view per sample (~200 cells) using Image J. pLV-THM, an empty vector cell line, and a mtx2kd-2 cell line carrying shRNA against Metaxin 2 served as controls (S2 Fig.). Significance was established using student’s t-test on the average circularity data. Data were normalized by setting the non-induced (-Dox) values to 1. * p<0.05, *** p<0.0001, n.s. not significant.