Fig 3. Multiple sequence alignment of deduced amino acid sequences of VcMATEs with selected MATE transporter orthologs.

Protein sequence alignment was performed using Clustalx. Formatting of aligned sequences was accomplished with the box shade program. Amino acids that are identical in all 8 proteins are highlighted in black and conservative substitutions are highlighted in gray. Alignment of all 56 Arabidopsis MATE proteins resulted in five short stretches containing conserved amino acids, which are highlighted by thin lines above the alignment and noted as D1–5. The residue E290 (TT12), constituting the cation-binding site in the pore, is highlighted by a red box.